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psbE-petL

LSC · 68946-70328 · 1383 bp

Back to Frangula

Pi 0.000723

MarkerSeek score 59.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1383
variable_sites: 1
pi: 0.000723
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCGGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1528 bp	1.0	79.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCGGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1530 bp	1.0	79.6
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCGGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1451 bp	1.0	78.8
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCGGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1453 bp	1.0	78.8
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GCGGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1537 bp	1.0	78.7