Hotspot

rps2-rpoC2

LSC · 17476-17678 · 203 bp

Back to Frangula

Pi 0.00493

MarkerSeek score 55.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 1
pi: 0.00493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GGTGCCATTCTAGGATTCCA	ATTCGAGCGGGAAATGAGAG	50.0	50.0	57.0	57.8	419 bp	1.0	70.1
rps2-rpoC2_p2	CCTGCTTCCATCATCTCTTCC	ATTCGAGCGGGAAATGAGAG	52.4	50.0	58.2	57.8	371 bp	1.0	69.2
rps2-rpoC2_p3	TGACCAAAATGAACTCCTGCT	ATTCGAGCGGGAAATGAGAG	42.9	50.0	57.8	57.8	386 bp	1.0	68.5
rps2-rpoC2_p4	ACTCCTGCTTCCATCATCTCT	ATTCGAGCGGGAAATGAGAG	47.6	50.0	58.2	57.8	374 bp	1.0	68.5
rps2-rpoC2_p5	TGAACTCCTGCTTCCATCATC	ATTCGAGCGGGAAATGAGAG	47.6	50.0	57.7	57.8	377 bp	1.0	68.2