Hotspot

trnK-UUU-rps16

LSC · 4574-5606 · 1033 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1033
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC ATTTCAAAGAAAGCGGGGGT 50.0 45.0 57.9 58.0 1213 bp 1.0 79.4
trnK-UUU-rps16_p2 GTCGCGGTCTTACAAACTCT ATTTCAAAGAAAGCGGGGGT 50.0 45.0 57.9 58.0 1212 bp 1.0 79.4
trnK-UUU-rps16_p3 AGTCGCGGTCTTACAAACTC TCCTGCAAAAAGGAGCTCAG 50.0 50.0 57.9 58.1 1254 bp 1.0 78.7
trnK-UUU-rps16_p4 GTCGCGGTCTTACAAACTCT TCCTGCAAAAAGGAGCTCAG 50.0 50.0 57.9 58.1 1253 bp 1.0 78.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT ATTTCAAAGAAAGCGGGGGT 50.0 45.0 58.2 58.0 1129 bp 1.0 78.6