Hotspot

matK-trnK-UUU

LSC · 3796-4536 · 741 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GAGGTCATGCTGCTGAGATC	AACGGTAGAGTACTCGGCTT	55.0	50.0	58.1	58.2	819 bp	1.0	77.6
matK-trnK-UUU_p2	GAGGTCATGCTGCTGAGATC	ACGGTAGAGTACTCGGCTTT	55.0	50.0	58.1	58.2	818 bp	1.0	77.6
matK-trnK-UUU_p3	ATAGGAGGTCATGCTGCTGA	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.2	58.2	823 bp	1.0	77.0
matK-trnK-UUU_p4	ATAGGAGGTCATGCTGCTGA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.2	58.2	822 bp	1.0	77.0
matK-trnK-UUU_p5	GAGGTCATGCTGCTGAGATC	GGGTTGCTAACTCAACGGTA	55.0	50.0	58.1	57.5	832 bp	1.0	75.3