Hotspot

rpl32-trnL-UAG

SSC · 114561-115186 · 626 bp

Back to Fragaria

Pi 0.00771
MarkerSeek score 67.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
626
variable_sites
25
pi
0.00771
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 722-769 bp 1.0 83.9
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 723-770 bp 1.0 83.9
rpl32-trnL-UAG_p3 GAAAGGGTATTGGGCAGCAT AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 733-780 bp 1.0 83.6
rpl32-trnL-UAG_p4 GAAAGGGTATTGGGCAGCAT CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 734-781 bp 1.0 83.6
rpl32-trnL-UAG_p5 AAAGGGTATTGGGCAGCATT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.7 58.0 732-779 bp 1.0 83.3