Hotspot

trnP-UGG-psaJ

LSC · 67709-68149 · 441 bp

Back to Fragaria

Pi 0.0161

MarkerSeek score 64.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 20
pi: 0.0161
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TCGAACCCGTGACATTTTGT	TACTTACTACCGGTGCCACA	45.0	50.0	58.0	58.1	249-571 bp	1.0	86.0
trnP-UGG-psaJ_p2	GGATGACAGGATTCGAACCC	TACTTACTACCGGTGCCACA	55.0	50.0	58.0	58.1	261-583 bp	1.0	86.0
trnP-UGG-psaJ_p3	GGGATGACAGGATTCGAACC	TACTTACTACCGGTGCCACA	55.0	50.0	58.0	58.1	262-584 bp	1.0	85.9
trnP-UGG-psaJ_p4	ACCCGTGACATTTTGTACCC	TACTTACTACCGGTGCCACA	50.0	50.0	58.1	58.1	245-567 bp	1.0	85.6
trnP-UGG-psaJ_p5	CCCGTGACATTTTGTACCCA	TACTTACTACCGGTGCCACA	50.0	50.0	58.1	58.1	244-566 bp	1.0	85.6