Hotspot

psbE-petL

LSC · 65692-66912 · 1221 bp

Back to Fragaria

Pi 0.00468

MarkerSeek score 59.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 22
pi: 0.00468
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGTCCG	50.0	55.0	57.8	58.2	1320-1336 bp	1.0	82.8
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGTCCG	45.0	55.0	58.2	58.2	1406-1422 bp	1.0	82.6
psbE-petL_p3	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGTCCG	50.0	55.0	58.2	58.2	1411-1427 bp	1.0	82.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGTCCG	55.0	55.0	58.2	58.2	1315-1331 bp	1.0	82.4
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGTCCGA	50.0	50.0	57.8	58.3	1319-1335 bp	1.0	82.1