Hotspot

ycf3-trnS-GGA

LSC · 45460-46327 · 868 bp

Back to Fragaria

Pi 0.00515
MarkerSeek score 59.40
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
868
variable_sites
18
pi
0.00515
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1042-2138 bp 1.0 71.4
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1043-2139 bp 1.0 71.4
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 992-2088 bp 1.0 71.1
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 993-2089 bp 1.0 71.1
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA AGTTCCAATGCTACGCCTTC 45.0 50.0 56.4 58.3 994-2090 bp 1.0 70.6