Hotspot

rps16-trnQ-UUG

LSC · 6175-7147 · 973 bp

Back to Fragaria

Pi 0.00668
MarkerSeek score 61.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
973
variable_sites
33
pi
0.00668
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 952-1133 bp 1.0 85.7
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 950-1131 bp 1.0 85.6
rps16-trnQ-UUG_p3 GATGTAAGACTCCACAGCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 966-1147 bp 1.0 85.3
rps16-trnQ-UUG_p4 GGATGTAAGACTCCACAGCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 967-1148 bp 1.0 85.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 934-1115 bp 1.0 84.8