Hotspot

matK-trnK-UUU

LSC · 3531-4263 · 733 bp

Back to Fragaria

Pi 0.00511

MarkerSeek score 58.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 16
pi: 0.00511
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CGATCATGAGCAAGGGCATA	ATTGTGGTGTGCCCTTCAAT	50.0	45.0	57.8	58.0	943-950 bp	1.0	84.1
matK-trnK-UUU_p2	AACCACGATCATGAGCAAGG	ATTGTGGTGTGCCCTTCAAT	50.0	45.0	58.3	58.0	948-955 bp	1.0	83.4
matK-trnK-UUU_p3	CGATCATGAGCAAGGGCATA	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.8	58.2	857-864 bp	1.0	82.7
matK-trnK-UUU_p4	CGATCATGAGCAAGGGCATA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.8	58.2	856-863 bp	1.0	82.7
matK-trnK-UUU_p5	CGATCATGAGCAAGGGCATA	TGTGGTGTGCCCTTCAATTT	50.0	45.0	57.8	58.2	941-948 bp	1.0	82.5