Hotspot

rpl32-trnP

Genome · 96988-98037 · 1050 bp

Back to Fossombronia

Pi 0.138

MarkerSeek score 82.20

Primer available yes

Label rpl32-trnP

Feature Metadata

label_name: rpl32-trnP
feature_type: igs
region: Genome
length_bp: 1050
variable_sites: 126
pi: 0.138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP_p1	GGATGGTAAACCGCTCGATT	CATCGCGCTCGGAATAGTTA	50.0	50.0	58.1	58.0	1727-1819 bp	1.0	90.5
rpl32-trnP_p2	GGATGGTAAACCGCTCGATT	TAACATCGCGCTCGGAATAG	50.0	50.0	58.1	58.0	1730-1822 bp	1.0	90.5
rpl32-trnP_p3	ACTCTTTCCCGGTGTGTTTC	CATCGCGCTCGGAATAGTTA	50.0	50.0	58.0	58.0	1923-2015 bp	1.0	90.4
rpl32-trnP_p4	ACTCTTTCCCGGTGTGTTTC	TAACATCGCGCTCGGAATAG	50.0	50.0	58.0	58.0	1926-2018 bp	1.0	90.4
rpl32-trnP_p5	ACTCTTTCCCGGTGTGTTTC	CTAACATCGCGCTCGGAATA	50.0	50.0	58.0	58.0	1927-2019 bp	1.0	90.3