Hotspot

trnM-CAU-trnT

Genome · 84824-85198 · 375 bp

Back to Fossombronia

Pi 0.0852
MarkerSeek score 80.50
Primer available yes
Label trnM-CAU-trnT

Feature Metadata

label_name
trnM-CAU-trnT
feature_type
igs
region
Genome
length_bp
375
variable_sites
31
pi
0.0852
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-trnT_p1 GGTGCTTTAACCATTCAGCC GACGACTTCCACCATGTCAA 50.0 50.0 57.3 57.8 453-521 bp 1.0 80.4
trnM-CAU-trnT_p2 GGGTGCTTTAACCATTCAGC GACGACTTCCACCATGTCAA 50.0 50.0 57.3 57.8 454-522 bp 1.0 80.3
trnM-CAU-trnT_p3 GGGTGCTTTAACCATTCAGC ACGACTTCCACCATGTCAAG 50.0 50.0 57.3 57.8 453-521 bp 1.0 80.3
trnM-CAU-trnT_p4 GGTGCTTTAACCATTCAGCC ACGACTTCCACCATGTCAAG 50.0 50.0 57.3 57.8 452-520 bp 1.0 80.3
trnM-CAU-trnT_p5 GGTGCTTTAACCATTCAGCC AACTGACGACTTCCACCATG 50.0 50.0 57.3 57.8 457-525 bp 1.0 80.2