Hotspot

ycf2-trnG-GCC

Genome · 37621-37959 · 339 bp

Back to Fossombronia

Pi 0.124

MarkerSeek score 78.10

Primer available yes

Label ycf2-trnG-GCC

Feature Metadata

label_name: ycf2-trnG-GCC
feature_type: igs
region: Genome
length_bp: 339
variable_sites: 40
pi: 0.124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnG-GCC_p1	GTTTGTTCCCACAGATCCGT	CTTTCTATCTGGGTCGGTGC	50.0	55.0	58.1	58.1	1225-1232 bp	1.0	85.7
ycf2-trnG-GCC_p2	GTTTGTTCCCACAGATCCGT	CCCCCACGATCGATGATTTT	50.0	50.0	58.1	58.0	1647-1650 bp	1.0	85.1
ycf2-trnG-GCC_p3	TAAGGGGGAGGAACGTACAG	CCCCCACGATCGATGATTTT	55.0	50.0	58.1	58.0	1818-1821 bp	1.0	84.4
ycf2-trnG-GCC_p4	GTTTGTTCCCACAGATCCGT	GAGCTCCAAATGGTCCAGAG	50.0	55.0	58.1	58.0	1109 bp	0.5	58.9
ycf2-trnG-GCC_p5	TAAGGGGGAGGAACGTACAG	GAGCTCCAAATGGTCCAGAG	55.0	55.0	58.1	58.0	1280 bp	0.5	58.6