Hotspot

rpl16

LSC · 84302-85573 · 1272 bp

Back to Forsythia

Pi 0.00322

MarkerSeek score 39.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1272
variable_sites: 10
pi: 0.00322
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACTTTCACCCCCAAGACTCA	TGCCCATTTGATATAATTGCT	50.0	33.3	58.5	53.6	1333-1337 bp	1.0	40.7
rpl16_p2	ACTTTCACCCCCAAGACTCA	TTGCCCATTTGATATAATTGCT	50.0	31.8	58.5	54.4	1334-1338 bp	1.0	40.7
rpl16_p3	AACTTTCACCCCCAAGACTC	TGCCCATTTGATATAATTGCT	50.0	33.3	57.3	53.6	1334-1338 bp	1.0	40.7
rpl16_p4	AACTTTCACCCCCAAGACTC	TTGCCCATTTGATATAATTGCT	50.0	31.8	57.3	54.4	1335-1339 bp	1.0	40.7
rpl16_p5	TCACCCCCAAGACTCATTTC	TGCCCATTTGATATAATTGCT	50.0	33.3	57.1	53.6	1329-1333 bp	1.0	40.7