Hotspot

petB

LSC · 78144-79496 · 1353 bp

Back to Forsythia

Pi 0.00275
MarkerSeek score 50.40
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1353
variable_sites
12
pi
0.00275
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 AGAGCTCGGATTTCTAGCCT TCCTCCTCCAAGTGATAAATGA 50.0 40.9 57.9 56.3 1479-1486 bp 1.0 50.0
petB_p2 TAAGAACCGCAAAGTCCTGG TCCTCCTCCAAGTGATAAATGA 50.0 40.9 57.8 56.3 1524-1531 bp 1.0 49.2
petB_p3 AGAGCTCGGATTTCTAGCCT TTCCTCCTCCAAGTGATAAATGA 50.0 39.1 57.9 57.0 1480-1487 bp 1.0 47.7
petB_p4 TAAGAACCGCAAAGTCCTGG TTCCTCCTCCAAGTGATAAATGA 50.0 39.1 57.8 57.0 1525-1532 bp 1.0 46.9
petB_p5 AGAGCTCGGATTTCTAGCCT TCCTCCTCCAAGTGATAAATGAT 50.0 39.1 57.9 56.8 1479-1486 bp 1.0 46.1