Hotspot

ndhF

SSC · 112831-115047 · 2217 bp

Back to Forsythia

Pi 0.00249

MarkerSeek score 47.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 13
pi: 0.00249
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGTTCTAAAAATAGCCCAGT	GAGGTAAGATCCGGTGAATCG	39.1	52.4	57.7	58.0	2360-2366 bp	1.0	46.3
ndhF_p2	AGCCCAGTTTTTGAAGATTCT	GAGGTAAGATCCGGTGAATCG	38.1	52.4	55.7	58.0	2345-2351 bp	1.0	45.6
ndhF_p3	TCGATTTACAGAGACAGGTTCT	GAGGTAAGATCCGGTGAATCG	40.9	52.4	56.6	58.0	2373-2379 bp	1.0	45.1
ndhF_p4	ACAGGTTCTAAAAATAGCCCAGT	AGGTAAGATCCGGTGAATCG	39.1	50.0	57.7	56.8	2359-2365 bp	1.0	44.9
ndhF_p5	TCCACTTTCGATTTACAGAGACA	GAGGTAAGATCCGGTGAATCG	39.1	52.4	57.5	58.0	2380-2386 bp	1.0	44.6