Hotspot

petA-psbJ

LSC · 66131-67058 · 928 bp

Back to Forsythia

Pi 0.00298

MarkerSeek score 50.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 7
pi: 0.00298
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1072-1075 bp	1.0	77.4
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	986-989 bp	1.0	76.3
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1069-1072 bp	1.0	76.2
petA-psbJ_p4	AGAAGGTTCAATTGGCCGAA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	985-988 bp	1.0	75.4
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1067-1070 bp	1.0	74.9