Hotspot

ycf1

SSC · 126332-132022 · 5691 bp

Back to Forsskaolea

Pi 0.342

MarkerSeek score 74.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5691
variable_sites: 1664
pi: 0.342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACTGCCGTTGTTGGTATCA	TTTTGCCCGTTGGTTTTTCC	50.0	45.0	58.2	58.0	1031 bp	0.5	58.2
ycf1_p2	GACTGCCGTTGTTGGTATCA	CGCAGTCCAAGCAGATTTTG	50.0	50.0	58.2	58.0	809 bp	0.5	58.2
ycf1_p3	GACTGCCGTTGTTGGTATCA	AGCAATATGGATACGGACGC	50.0	50.0	58.2	57.9	1231 bp	0.5	57.8
ycf1_p4	ACTGCCGTTGTTGGTATCAA	TTTTGCCCGTTGGTTTTTCC	45.0	45.0	57.7	58.0	1030 bp	0.5	57.7
ycf1_p5	ACTGCCGTTGTTGGTATCAA	CGCAGTCCAAGCAGATTTTG	45.0	50.0	57.7	58.0	808 bp	0.5	57.7