Hotspot

rps15-ycf1

SSC · 125893-126331 · 439 bp

Back to Forsskaolea

Pi 0.455

MarkerSeek score 78.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 439
variable_sites: 180
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGACTCGTCAAATTAACGCG	TATTGAAAGGCGCGAACTGA	50.0	45.0	58.0	57.9	1337 bp	0.5	59.0
rps15-ycf1_p2	AACACCATGCATTGATGGGT	TATTGAAAGGCGCGAACTGA	45.0	45.0	58.1	57.9	1503 bp	0.5	58.8
rps15-ycf1_p3	CGACTCGTCAAATTAACGCG	TGATACCAACAACGGCAGTC	50.0	50.0	58.0	58.2	806 bp	0.5	58.1
rps15-ycf1_p4	CGACTCGTCAAATTAACGCG	TTCAATTGCGATGGAGTGGT	50.0	45.0	58.0	57.8	1443 bp	0.5	58.1
rps15-ycf1_p5	AACACCATGCATTGATGGGT	TGATACCAACAACGGCAGTC	45.0	50.0	58.1	58.2	972 bp	0.5	58.0