Hotspot

ndhE-ndhG

SSC · 120318-120545 · 228 bp

Back to Forsskaolea

Pi 0.482

MarkerSeek score 74.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 228
variable_sites: 93
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCCTCTGTTCTTGGAGCAGA	GCGGCCCAACTCCTTATTTA	50.0	50.0	58.0	58.0	1303 bp	0.5	59.5
ndhE-ndhG_p2	CTTAGCTTTACACCCGTCCC	GCGGCCCAACTCCTTATTTA	55.0	50.0	58.0	58.0	1274 bp	0.5	59.5
ndhE-ndhG_p3	TTACACCCGTCCCAAGGTAT	GCGGCCCAACTCCTTATTTA	50.0	50.0	58.0	58.0	1267 bp	0.5	59.3
ndhE-ndhG_p4	TCCTCTGTTCTTGGAGCAGA	TTTGGACCGTTGGTAATGGG	50.0	50.0	58.0	58.1	1206 bp	0.5	59.2
ndhE-ndhG_p5	CTTAGCTTTACACCCGTCCC	TTTGGACCGTTGGTAATGGG	55.0	50.0	58.0	58.1	1177 bp	0.5	59.1