Hotspot

psbG

LSC · 51318-52028 · 711 bp

Back to Forestiera

Pi 0.003

MarkerSeek score 59.40

Primer available yes

Label psbG

Feature Metadata

label_name: psbG
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 5
pi: 0.003
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG_p1	TGAAGATTCACCCACTACTTGT	GTTTATGCATGGCGAAAGGG	40.9	50.0	57.0	57.8	820-853 bp	1.0	58.2
psbG_p2	TGAAGATTCACCCACTACTTGT	GGGGCATTGGAATGGTCTTA	40.9	50.0	57.0	57.5	802-835 bp	1.0	56.3
psbG_p3	TGAAGATTCACCCACTACTTGTT	GTTTATGCATGGCGAAAGGG	39.1	50.0	57.7	57.8	820-853 bp	1.0	55.6
psbG_p4	ATGAAGATTCACCCACTACTTGT	GTTTATGCATGGCGAAAGGG	39.1	50.0	57.5	57.8	821-854 bp	1.0	53.9
psbG_p5	TGAAGATTCACCCACTACTTGTT	GGGGCATTGGAATGGTCTTA	39.1	50.0	57.7	57.5	802-835 bp	1.0	53.7