Hotspot

rpoB-trnC

LSC · 27980-29187 · 1208 bp

Back to Forestiera

Pi 0.00535

MarkerSeek score 53.70

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1208
variable_sites: 13
pi: 0.00535
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1263-1291 bp	1.0	73.8
rpoB-trnC_p2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1268-1296 bp	1.0	73.2
rpoB-trnC_p3	CTCATTTCCATCCCCGAGC	AAAGGATTTGCAGTCCTCCG	57.9	50.0	58.0	58.2	1244-1272 bp	1.0	73.1
rpoB-trnC_p4	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1262-1290 bp	1.0	72.7
rpoB-trnC_p5	ATTCCCTCATTTCCATCCCC	AAAGGATTTGCAGTCCTCCG	50.0	50.0	56.9	58.2	1249-1277 bp	1.0	72.5