Hotspot

atpH-atpI

LSC · 14661-15728 · 1068 bp

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Pi 0.0088

MarkerSeek score 49.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1068
variable_sites: 25
pi: 0.0088
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCAAACAAGAGGTCCAAGG	TGCTTTGGCTTTACTCACGT	50.0	45.0	58.0	58.0	1409-1709 bp	0.833333	78.2
atpH-atpI_p2	AGCAAACAAGAGGTCCAAGG	GGCAAATAGGGGGTTTCCAA	50.0	50.0	58.0	58.1	1740-2040 bp	0.833333	77.9
atpH-atpI_p3	AGCAAACAAGAGGTCCAAGG	TTGGCAAATAGGGGGTTTCC	50.0	50.0	58.0	58.1	1742-2042 bp	0.833333	77.9
atpH-atpI_p4	AGCAAACAAGAGGTCCAAGG	ATAGGGGGTTTCCAAGTCCA	50.0	50.0	58.0	57.9	1735-2035 bp	0.833333	77.8
atpH-atpI_p5	AGCAAACAAGAGGTCCAAGG	TAGGGGGTTTCCAAGTCCAT	50.0	50.0	58.0	57.9	1734-2034 bp	0.833333	77.8