Hotspot

rps16-trnQ

LSC · 6437-8091 · 1655 bp

Back to Forestiera

Pi 0.00729
MarkerSeek score 45.50
Primer available yes
Label rps16-trnQ

Feature Metadata

label_name
rps16-trnQ
feature_type
igs
region
LSC
length_bp
1655
variable_sites
30
pi
0.00729
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_p1 CGGATCTGTCCTTCAAGTCG GGTTCGAATCCTTCCGTCC 55.0 57.9 58.1 57.9 1732-1742 bp 1.0 73.0
rps16-trnQ_p2 CGGATCTGTCCTTCAAGTCG GTTCGAATCCTTCCGTCCC 55.0 57.9 58.1 57.9 1731-1741 bp 1.0 73.0
rps16-trnQ_p3 ATCTGTCCTTCAAGTCGCAC GGTTCGAATCCTTCCGTCC 50.0 57.9 57.9 57.9 1729-1739 bp 1.0 73.0
rps16-trnQ_p4 ATCTGTCCTTCAAGTCGCAC GTTCGAATCCTTCCGTCCC 50.0 57.9 57.9 57.9 1728-1738 bp 1.0 73.0
rps16-trnQ_p5 AAGTCGCACGTTGCTTTCTA GTTCGAATCCTTCCGTCCC 45.0 57.9 58.1 57.9 1717-1727 bp 1.0 72.6