Hotspot

psbA

LSC · 304-1368 · 1065 bp

Back to Fordiophyton

Pi 0.00208

MarkerSeek score 43.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1065
variable_sites: 10
pi: 0.00208
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AAACTCGTCTAAATTCAGACT	AGAATTCGTGTGCTTGGGAG	33.3	50.0	52.6	58.2	1147-1150 bp	1.0	40.9
psbA_p2	AAACTCGTCTAAATTCAGACT	AGAGAATTCGTGTGCTTGGG	33.3	50.0	52.6	58.2	1149-1152 bp	1.0	40.9
psbA_p3	AAACTCGTCTAAATTCAGACT	GAGAATTCGTGTGCTTGGGA	33.3	50.0	52.6	58.2	1148-1151 bp	1.0	40.9
psbA_p4	AAACTCGTCTAAATTCAGACT	TGGGAGTCCCTGATGATGAA	33.3	50.0	52.6	57.7	1133-1136 bp	1.0	40.9
psbA_p5	AAACTCGTCTAAATTCAGACT	TTGGGAGTCCCTGATGATGA	33.3	50.0	52.6	57.7	1134-1137 bp	1.0	40.9