Hotspot

ndhH-rps15

SSC · 124497-124624 · 128 bp

Back to Fordiophyton

Pi 0.0307
MarkerSeek score 75.50
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
128
variable_sites
15
pi
0.0307
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 TGCATTGATGGGTGATGAGG CGGGAATCAAAAACGCGTTA 50.0 45.0 57.9 57.4 198-230 bp 1.0 85.4
ndhH-rps15_p2 GCATTGATGGGTGATGAGGT CGGGAATCAAAAACGCGTTA 50.0 45.0 57.9 57.4 197-229 bp 1.0 85.4
ndhH-rps15_p3 TGCATTGATGGGTGATGAGG ATTCGGGAATCAAAAACGCG 50.0 45.0 57.9 57.7 201-233 bp 0.909091 83.3
ndhH-rps15_p4 GCATTGATGGGTGATGAGGT ATTCGGGAATCAAAAACGCG 50.0 45.0 57.9 57.7 200-232 bp 0.909091 83.3
ndhH-rps15_p5 TAAGACGAAGAACACCGTGC ATTCGGGAATCAAAAACGCG 50.0 45.0 58.2 57.7 218-250 bp 0.909091 81.6