Hotspot

ccsA-ndhD

SSC · 116680-116999 · 320 bp

Back to Fordiophyton

Pi 0.0154

MarkerSeek score 74.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 320
variable_sites: 24
pi: 0.0154
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTGGCTTCTATGGGCTTTC	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.9	57.6	461-479 bp	1.0	83.4
ccsA-ndhD_p2	TGTGGCTTCTATGGGCTTTC	CAGTTGAGAAGGTCGAAGCT	50.0	50.0	57.9	57.6	460-478 bp	1.0	83.4
ccsA-ndhD_p3	GATTGTGGCTTCTATGGGCT	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.6	57.6	464-482 bp	1.0	81.6
ccsA-ndhD_p4	GATTGTGGCTTCTATGGGCT	CAGTTGAGAAGGTCGAAGCT	50.0	50.0	57.6	57.6	463-481 bp	1.0	81.5
ccsA-ndhD_p5	GTGGCTTCTATGGGCTTTCT	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.6	57.6	460-478 bp	1.0	81.0