Hotspot

rbcL-accD

LSC · 57207-57806 · 600 bp

Back to Fordiophyton

Pi 0.0124

MarkerSeek score 71.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 28
pi: 0.0124
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	ATCCTCACTTCCACGAGTGT	50.0	50.0	57.9	58.4	691-806 bp	1.0	83.8
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	602-711 bp	0.818182	76.8
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	GAATTTCTCGCCGCTAGACA	50.0	50.0	57.9	58.1	708-729 bp	0.272727	51.3
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	GAATTTCTCGCCGCTAGACA	55.0	50.0	57.7	58.1	724-745 bp	0.272727	49.8
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	ATTTCTCGCCGCTAGACAAC	50.0	50.0	57.9	58.4	706-727 bp	0.272727	49.0