Hotspot

ndhC-trnV-UAC

LSC · 51209-51890 · 682 bp

Back to Fordiophyton

Pi 0.0252

MarkerSeek score 71.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 682
variable_sites: 67
pi: 0.0252
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CAATGCTCCTTTTCGCCATG	AACACTTTGGTATTGCCCGT	50.0	45.0	58.1	58.0	1038-1327 bp	1.0	88.3
ndhC-trnV-UAC_p2	ATCCGACTGTCTAGGACTCG	AACACTTTGGTATTGCCCGT	55.0	45.0	58.0	58.0	1321-1616 bp	1.0	87.9
ndhC-trnV-UAC_p3	CCGACTGTCTAGGACTCGAT	AACACTTTGGTATTGCCCGT	55.0	45.0	58.0	58.0	1319-1614 bp	1.0	87.9
ndhC-trnV-UAC_p4	ATCCGACTGTCTAGGACTCG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	1103-1398 bp	0.545455	66.7
ndhC-trnV-UAC_p5	CCGACTGTCTAGGACTCGAT	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	1101-1396 bp	0.545455	66.7