Hotspot

psaA-ycf3

LSC · 42275-42870 · 596 bp

Back to Fordiophyton

Pi 0.00714

MarkerSeek score 71.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 596
variable_sites: 17
pi: 0.00714
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	734-776 bp	1.0	84.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	716-758 bp	1.0	84.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.9	57.8	660-702 bp	1.0	83.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	659-701 bp	1.0	83.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	730-772 bp	1.0	82.9