Hotspot

atpF-atpH

LSC · 13087-13603 · 517 bp

Back to Fordiophyton

Pi 0.0109

MarkerSeek score 71.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 517
variable_sites: 22
pi: 0.0109
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GGATTGGTTGTCGCTTTAGC	50.0	50.0	57.9	57.5	534-634 bp	1.0	81.8
atpF-atpH_p2	GGCCCAAGGAAAGGAAAGAA	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.0	57.5	502-602 bp	0.909091	78.7
atpF-atpH_p3	GGCCCAAGGAAAGGAAAGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	567-667 bp	0.909091	77.3
atpF-atpH_p4	GGCCCAAGGAAAGGAAAGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	568-668 bp	0.909091	77.1
atpF-atpH_p5	GGCCCAAGGAAAGGAAAGAA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	569-669 bp	0.909091	77.1