Hotspot

ycf1

SSC · 125750-131320 · 5571 bp

Back to Fontanesia

Pi 0.0101

MarkerSeek score 51.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5571
variable_sites: 56
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTCATTCTGGGAATATTAATGA	TCTCCAGGTTGGACAAATTCT	29.2	42.9	53.5	56.8	262-298 bp	1.0	45.4
ycf1_p2	TCTTCATTCTGGGAATATTAATGA	TCCAGGTTGGACAAATTCTTCA	29.2	40.9	53.5	57.8	260-296 bp	1.0	45.4
ycf1_p3	TTCTTCATTCTGGGAATATTAATGA	TCTCCAGGTTGGACAAATTCT	28.0	42.9	54.3	56.8	263-299 bp	1.0	45.4
ycf1_p4	TTCTTCATTCTGGGAATATTAATGA	TCCAGGTTGGACAAATTCTTCA	28.0	40.9	54.3	57.8	261-297 bp	1.0	45.4
ycf1_p5	TTTTTCTTCATTCTGGGAATATT	TCTCCAGGTTGGACAAATTCT	26.1	42.9	52.3	56.8	266-302 bp	1.0	45.4