Hotspot

petD

LSC · 79243-80443 · 1201 bp

Back to Fontanesia

Pi 0.00667

MarkerSeek score 49.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1201
variable_sites: 8
pi: 0.00667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGAGTTGAAGGGAATTCTCCG	GTCGTGTTATTTCACAATTGAATCA	52.4	32.0	58.4	56.9	1263-1264 bp	1.0	40.6
petD_p2	GGAGTTGAAGGGAATTCTCCG	GTCGTGTTATTTCACAATTGA	52.4	33.3	58.4	52.8	1263-1264 bp	1.0	40.6
petD_p3	GAGTTGAAGGGAATTCTCCGA	GTCGTGTTATTTCACAATTGAATCA	47.6	32.0	57.4	56.9	1262-1263 bp	1.0	40.6
petD_p4	GGAGTTGAAGGGAATTCTCCG	GTCGTGTTATTTCACAATTGAA	52.4	31.8	58.4	53.7	1263-1264 bp	1.0	40.6
petD_p5	GGAGTTGAAGGGAATTCTCCG	TCGTGTTATTTCACAATTGAATCA	52.4	29.2	58.4	55.6	1262-1263 bp	1.0	40.6