Hotspot

petB

LSC · 77714-79060 · 1347 bp

Back to Fontanesia

Pi 0.00891

MarkerSeek score 50.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1347
variable_sites: 12
pi: 0.00891
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGAGCTCGGATTTCTAGCCT	TTTCCGGAGTTGCAACTACC	50.0	50.0	57.9	58.1	2156-2174 bp	1.0	79.4
petB_p2	AGAGCTCGGATTTCTAGCCT	TGGATCTTACGGAGTCTGCT	50.0	50.0	57.9	57.9	1805-1823 bp	1.0	79.2
petB_p3	GTCGCAATGGCTCTATTTGC	TTTCCGGAGTTGCAACTACC	50.0	50.0	57.9	58.1	2296-2314 bp	1.0	79.1
petB_p4	GTCGCAATGGCTCTATTTGC	TGGATCTTACGGAGTCTGCT	50.0	50.0	57.9	57.9	1945-1963 bp	1.0	78.8
petB_p5	AGAGCTCGGATTTCTAGCCT	CGGAGTTGCAACTACCCATT	50.0	50.0	57.9	58.2	2152-2170 bp	1.0	78.8