Hotspot

ndhF

SSC · 112401-114617 · 2217 bp

Back to Fontanesia

Pi 0.0104

MarkerSeek score 49.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 23
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.0	58.0	2622-2623 bp	1.0	72.4
ndhF_p2	GAGATCCGCCGAAGAAGATC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2486-2487 bp	1.0	72.2
ndhF_p3	CAGGAACAAGAGAGATCCGC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.1	58.0	2497-2498 bp	1.0	72.2
ndhF_p4	CGCCGAAGAAGATCCTTCTC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.2	58.0	2480-2481 bp	1.0	71.5
ndhF_p5	ACAGCTTCCGAAATGAAGGG	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.2	58.0	2524-2525 bp	1.0	71.3