Hotspot

trnL-UAG-ccsA

SSC · 116197-116286 · 90 bp

Back to Fontanesia

Pi 0.0333

MarkerSeek score 69.60

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 90
variable_sites: 3
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	ACAATTGAAACGATCGAAAAAGA	50.0	30.4	57.9	55.7	213 bp	1.0	41.1
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	ACAATTGAAACGATCGAAAAAGA	45.0	30.4	57.8	55.7	220 bp	1.0	41.1
trnL-UAG-ccsA_p3	GTAGACACGCTGCTCTTAGG	ACAATTGAAACGATCGAAAAAGA	55.0	30.4	57.8	55.7	214 bp	1.0	41.1
trnL-UAG-ccsA_p4	GAGTTCAAGACAAGCCGCTA	ACAATTGAAACGATCGAAAAAGA	50.0	30.4	57.9	55.7	245 bp	1.0	41.0
trnL-UAG-ccsA_p5	ACAAGCCGCTATGGTGAAAT	ACAATTGAAACGATCGAAAAAGA	45.0	30.4	57.9	55.7	236 bp	1.0	41.0