Hotspot

ndhF-rpl32

SSC · 114618-115114 · 497 bp

Back to Fontanesia

Pi 0.00634

MarkerSeek score 63.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 497
variable_sites: 3
pi: 0.00634
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAAGGTATGATCCACGC	GCTGTCCCATATCCCTTCCT	50.0	55.0	58.1	58.6	597-621 bp	1.0	78.6
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	GCTGTCCCATATCCCTTCCT	50.0	55.0	58.0	58.6	654-678 bp	1.0	78.5
ndhF-rpl32_p3	CGAAAGGTATGATCCACGCA	GCTGTCCCATATCCCTTCCT	50.0	55.0	58.1	58.6	596-620 bp	1.0	78.5
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	GCTGTCCCATATCCCTTCCT	45.0	55.0	58.0	58.6	652-676 bp	1.0	78.4
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	GCTGTCCCATATCCCTTCCT	45.0	55.0	58.0	58.6	653-677 bp	1.0	78.4