Hotspot

ycf1-ndhF

SSC · 112370-112400 · 31 bp

Back to Fontanesia

Pi 0.0968

MarkerSeek score 80.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 31
variable_sites: 3
pi: 0.0968
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TCCGAGTGAATGGAAAGGAA	AATATGTAGGGGGTGGACGT	45.0	50.0	56.5	57.8	234-235 bp	1.0	67.2
ycf1-ndhF_p2	TCGATTTACAGAGACAGGCTC	AATATGTAGGGGGTGGACGT	47.6	50.0	57.2	57.8	191-192 bp	1.0	65.5
ycf1-ndhF_p3	ATCCGAGTGAATGGAAAGGA	AATATGTAGGGGGTGGACGT	45.0	50.0	56.2	57.8	235-236 bp	1.0	65.4
ycf1-ndhF_p4	TCCGAGTGAATGGAAAGGAAA	AATATGTAGGGGGTGGACGT	42.9	50.0	57.2	57.8	234-235 bp	1.0	65.3
ycf1-ndhF_p5	ATCCGAGTGAATGGAAAGGAA	AATATGTAGGGGGTGGACGT	42.9	50.0	57.0	57.8	235-236 bp	1.0	63.5