Hotspot

rpl33-rps18

LSC · 70603-70771 · 169 bp

Back to Fontanesia

Pi 0.0244

MarkerSeek score 65.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 4
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GGCACAATACGCCTAATCGA	CAATTCGATCCCCCGATTGA	50.0	50.0	58.1	57.7	335-340 bp	1.0	79.0
rpl33-rps18_p2	GGCACAATACGCCTAATCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	336-341 bp	1.0	79.0
rpl33-rps18_p3	ACTCAAAAGAACCGGCACAA	CAATTCGATCCCCCGATTGA	45.0	50.0	58.2	57.7	348-353 bp	1.0	78.1
rpl33-rps18_p4	ACTCAAAAGAACCGGCACAA	TCAATTCGATCCCCCGATTG	45.0	50.0	58.2	57.7	349-354 bp	1.0	78.1
rpl33-rps18_p5	GGCACAATACGCCTAATCGA	TTCGATCCCCCGATTGAATC	50.0	50.0	58.1	57.5	332-337 bp	1.0	76.7