Hotspot

psaJ-rpl33

LSC · 69955-70401 · 447 bp

Back to Fontanesia

Pi 0.0135

MarkerSeek score 52.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 6
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	CAAAATCACCGTTACTCGCG	45.0	50.0	58.1	57.9	517-518 bp	1.0	79.4
psaJ-rpl33_p2	TGCGTTGACATTCCCCTTTT	CAAAATCACCGTTACTCGCG	45.0	50.0	58.3	57.9	516-517 bp	1.0	77.6
psaJ-rpl33_p3	ATGCGTTGACATTCCCCTTT	CCAAAATCACCGTTACTCGC	45.0	50.0	58.1	57.5	518-519 bp	1.0	76.3
psaJ-rpl33_p4	GATGCGTTGACATTCCCCTT	CAAAATCACCGTTACTCGCG	50.0	50.0	58.5	57.9	518-519 bp	1.0	75.7
psaJ-rpl33_p5	ATGCGTTGACATTCCCCTTT	ACACAACCGGTACATTCCAA	45.0	45.0	58.1	57.4	534-535 bp	1.0	75.3