Hotspot

psbE-petL

LSC · 67441-68595 · 1155 bp

Back to Fontanesia

Pi 0.0156

MarkerSeek score 59.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 18
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1345-1347 bp	1.0	73.3
psbE-petL_p2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1358-1360 bp	1.0	73.0
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1268-1270 bp	1.0	72.5
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1354-1356 bp	1.0	72.3
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1353-1355 bp	1.0	72.2