Hotspot

ycf4-cemA

LSC · 63014-63892 · 879 bp

Back to Fontanesia

Pi 0.0203

MarkerSeek score 66.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 15
pi: 0.0203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.0	58.1	915-1055 bp	1.0	84.4
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	914-1054 bp	1.0	84.2
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	913-1053 bp	1.0	84.2
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	840-980 bp	1.0	83.8
ycf4-cemA_p5	GAACAAAAAGCCGCCGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	869-1009 bp	1.0	82.9