Hotspot

atpA-atpF

LSC · 12703-12762 · 60 bp

Back to Fontanesia

Pi 0.0508

MarkerSeek score 82.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 3
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TTGGGGGCGATGAAAGAAAT	50.0	45.0	58.0	57.8	205-206 bp	1.0	79.4
atpA-atpF_p2	ATGCCATCGCCTACTTGAAG	ATATTGACATGTTGGGGGCG	50.0	50.0	58.0	58.3	216-217 bp	1.0	78.7
atpA-atpF_p3	CAATGCCATCGCCTACTTGA	TTGGGGGCGATGAAAGAAAT	50.0	45.0	58.3	57.8	207-208 bp	1.0	77.0
atpA-atpF_p4	AATGCCATCGCCTACTTGAA	TTGGGGGCGATGAAAGAAAT	45.0	45.0	57.6	57.8	206-207 bp	1.0	76.4
atpA-atpF_p5	CAATGCCATCGCCTACTTGA	ATATTGACATGTTGGGGGCG	50.0	50.0	58.3	58.3	218-219 bp	1.0	76.3