Hotspot

trnK-UUU-rps16

LSC · 4398-5285 · 888 bp

Back to Fontanesia

Pi 0.0102
MarkerSeek score 65.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
888
variable_sites
9
pi
0.0102
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATTCCTCGCTTCATCCA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.1 58.2 1060-1082 bp 1.0 79.0
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1012-1034 bp 1.0 78.1
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1011-1033 bp 1.0 78.1
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 1026-1048 bp 1.0 77.4
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 1021-1043 bp 1.0 77.2