Hotspot

ycf1

SSC · 131078-136891 · 5814 bp

Pi 0.0327

MarkerSeek score 63.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5814
variable_sites: 174
pi: 0.0327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTCCAGTCCCCAAACTGT	TGAGCGGAAAACAGATGCAT	50.0	45.0	57.9	58.2	1092-1095 bp	1.0	84.6
ycf1_p2	TTTGTGCCAAGGTTTCAGGT	TGAGCGGAAAACAGATGCAT	45.0	45.0	58.1	58.2	1003-1006 bp	1.0	84.5
ycf1_p3	CATCCCCTCATTAGTTGCCC	TGAGCGGAAAACAGATGCAT	55.0	45.0	58.0	58.2	747 bp	0.5	58.5
ycf1_p4	GTTGCCCATGTGACAGGTTA	TGAGCGGAAAACAGATGCAT	50.0	45.0	58.1	58.2	734 bp	0.5	57.8
ycf1_p5	CATCCCCTCATTAGTTGCCC	ACACATGCCAAAGTGATGGA	55.0	45.0	58.0	57.7	1192 bp	0.5	57.6