Hotspot

clpP

LSC · 77491-79506 · 2016 bp

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Pi 0.0165
MarkerSeek score 57.40
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2016
variable_sites
33
pi
0.0165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 TGGGCTGGTTTAGATTGATCC TGTTACGTTTCCACATCAAAGT 47.6 36.4 57.7 56.5 2183-2188 bp 1.0 43.3
clpP_p2 GGGCTGGTTTAGATTGATCCT TGTTACGTTTCCACATCAAAGT 47.6 36.4 57.4 56.5 2182-2187 bp 1.0 41.7
clpP_p3 TGGGCTGGTTTAGATTGATCC TTGTTACGTTTCCACATCAAAGT 47.6 34.8 57.7 57.2 2184-2189 bp 1.0 41.7
clpP_p4 GGGCTGGTTTAGATTGATCCT TTGTTACGTTTCCACATCAAAGT 47.6 34.8 57.4 57.2 2183-2188 bp 1.0 41.7
clpP_p5 TGGGCTGGTTTAGATTGATCC TGTTACGTTTCCACATCAAAG 47.6 38.1 57.7 54.7 2183-2188 bp 1.0 41.7