Hotspot

ccsA-ndhD

SSC · 122663-122915 · 253 bp

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Pi 0.0356
MarkerSeek score 67.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
253
variable_sites
9
pi
0.0356
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATTCGGCAATTGTAGCGTCT CCCGATTTCGTTCTGTCACT 45.0 50.0 58.0 57.9 438 bp 1.0 80.8
ccsA-ndhD_p2 ATTCGGCAATTGTAGCGTCT TGTACCCCGATTTCGTTCTG 45.0 50.0 58.0 57.6 443 bp 1.0 78.4
ccsA-ndhD_p3 ATTCGGCAATTGTAGCGTCT GTACCCCGATTTCGTTCTGT 45.0 50.0 58.0 57.6 442 bp 1.0 78.4
ccsA-ndhD_p4 ATTCGGCAATTGTAGCGTCT TACCCCGATTTCGTTCTGTC 45.0 50.0 58.0 57.3 441 bp 1.0 76.2
ccsA-ndhD_p5 TTCGGCAATTGTAGCGTCTA CCCGATTTCGTTCTGTCACT 45.0 50.0 57.3 57.9 437 bp 1.0 75.7