Hotspot

trnN-GUU-ndhF

IRb · 115978-117544 · 1567 bp

Back to Fockea

Pi 0.00627
MarkerSeek score 66.00
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1567
variable_sites
9
pi
0.00627
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 AGGATTCGAACCTACGACCA CGGGCGCATCTCTTCTTATC 50.0 55.0 58.2 58.6 1582-1714 bp 1.0 78.6
trnN-GUU-ndhF_p2 CCCCAAGTAGGATTCGAACC CGGGCGCATCTCTTCTTATC 55.0 55.0 57.7 58.6 1590-1722 bp 1.0 77.2
trnN-GUU-ndhF_p3 AGGATTCGAACCTACGACCA GGGCGCATCTCTTCTTATCT 50.0 50.0 58.2 57.2 1581-1713 bp 1.0 76.5
trnN-GUU-ndhF_p4 AGGATTCGAACCTACGACCA ATCAAATATGTAGGGGGCGG 50.0 50.0 58.2 57.1 1599-1731 bp 1.0 76.1
trnN-GUU-ndhF_p5 CCCCAAGTAGGATTCGAACC GGGCGCATCTCTTCTTATCT 55.0 50.0 57.7 57.2 1589-1721 bp 1.0 75.2