Hotspot

petA-psbJ

LSC · 70622-71301 · 680 bp

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Pi 0.015
MarkerSeek score 69.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
680
variable_sites
10
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GCCGATACTACTGGAAGGAT 50.0 50.0 58.0 56.2 919-930 bp 1.0 67.2
petA-psbJ_p2 CATTACGTGTCCAAGGCCTT GCCGATACTACTGGAAGGAT 50.0 50.0 58.2 56.2 916-927 bp 1.0 66.1
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC GCCGATACTACTGGAAGGATT 50.0 47.6 58.0 57.0 919-930 bp 1.0 65.3
petA-psbJ_p4 ATCCATTACGTGTCCAAGGC GCCGATACTACTGGAAGGATTC 50.0 50.0 58.0 58.1 919-930 bp 1.0 64.9
petA-psbJ_p5 TTACGTGTCCAAGGCCTTTT GCCGATACTACTGGAAGGAT 45.0 50.0 57.6 56.2 914-925 bp 1.0 64.8