Hotspot

ndhC-trnV-UAC

LSC · 57170-58645 · 1476 bp

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Pi 0.0107
MarkerSeek score 67.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1476
variable_sites
15
pi
0.0107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGTTAATCGGGGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1546-1621 bp 1.0 82.2
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1532-1607 bp 1.0 82.1
ndhC-trnV-UAC_p3 GTCCTTTGTTAATCGGGGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1545-1620 bp 1.0 79.4
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1531-1606 bp 1.0 79.3
ndhC-trnV-UAC_p5 TGGTCCTTTGTTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1548-1623 bp 1.0 79.2